Digital transformation has disrupted various healthcare domains, yet we are just starting to witness the enrollment of digital solutions enabled by technologies such as artificial intelligence and machine learning. Stroke is one of the leading causes of death and permanent disability globally. Research evidence suggests a need for newer technology-enabled collaborative service models that will not only improve stroke care but are economically viable for stakeholders (i.e., service providers, patients, and payers). A new collaborative service model equally requires the development of new collaborative business models in the stroke care cycle. In a large-scale research ecosystem in Finland, the stroke care pathway is utilized to identify the scope of digitalization in early stroke diagnosis, treatment, rehabilitation, and secondary prevention. This study presents how the stroke care pathway has been used as the basis for new collaborative business models and healthcare service innovation.