The economic development process associated with entrepreneurial ecosystems comprises different approaches and its understanding is vital for regional growth. The Polytechnic Institute of Porto is boosting its entrepreneurial ecosystem, to reinforce its action as an agent of economic and social development in the regions where it operates. The objective of the work was to propose the criteriafor the construction of an analysis model that allows the identification of regional "strategic bets" that will support the development of proposals for the provision of support services that integrate with the regional business base and incorporate "Decision Intelligence" to performance of the "Entrepreneurial Regional Observatory of the Porto Startup Network." The methodology used was an exploratory research and, at the end, the initiatives taken and the results are presented.