Collaborative Networks have shown to have a high potential as drivers of value creation; therefore, it is increasingly frequent to observe how organisations and governments are implementing collaborative networking to support their innovation processes and foster entrepreneurship in benefit to society. Consequently, it is observed that these implementations can contribute to accelerating the development of weaker local and regional economies, especially in developing countries. In this paper, it is presented the Open Innovation Hub of México City of the ECOS Network and how by applying collaborative networking, it is possible to foster both innovation and entrepreneurship. Also, the Learning Centre for Entrepreneurship in Mexico City as part of this Hub is described. Finally, a case study with an imparted teaching-learning program is presented. And so, there are described how by using the concepts of Open Innovation and Education 4.0 are designed and implemented specific teaching-learning programs to encourage the creation of new enterprises.