The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI - Computer and Computing Technologies in Agriculture IX - Part II Access content directly
Conference Papers Year : 2016

The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI

Weiqing Wang
  • Function : Author
  • PersonId : 1012122

Abstract

Objective: to establish a quick molecular detection method in Beijing, which can prevent the occurrence of cucumber target spot disease from the source. Methods and results: The DNA band of Corynespora cassiicola had been obtained by PCR and sequenced. Using DNAman software and NCBI database to analyze the sequence, the results showed that the obtained DNA was that of Corynespora cassiicola on Cucumber. A specific primers CCC1/CCC2 were obtained by DNAman software and NCBI database. It was also proved to can be used to distinguish Corynespora cassiicola from other pathogenic fungi using DNAman software and NCBI database.
Fichier principal
Vignette du fichier
434298_1_En_26_Chapter.pdf (603.51 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-01614171 , version 1 (10-10-2017)

Licence

Attribution

Identifiers

Cite

Weiqing Wang. The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI. 9th International Conference on Computer and Computing Technologies in Agriculture (CCTA), Sep 2015, Beijing, China. pp.248-258, ⟨10.1007/978-3-319-48354-2_26⟩. ⟨hal-01614171⟩
151 View
245 Download

Altmetric

Share

Gmail Facebook X LinkedIn More