This study revealed the influence of city expansion on the agro-ecological risks through the analysis and prediction of city expansion in different periods and study on the change of risk and pressure on the regional agricultural eco-environment. The city expansion of Selangor, Malaysia (as a case) was predicted based on relevant spatial and attribute data as well as simulation prediction models of city expansion. Subsequently, the ecological risk and pressure in the study area as well as on regional agricultural land use was assessed through the realization of factors of SERA Model. The results showed that the risk and pressure on agricultural land use was consistent with the level of the urbanization. With the expansion of urban area, the ecological risk on agricultural land use in the study area became greater. The risk and pressure on agricultural land use with city expansion was well analyzed with SERA model.